CTS1076: Cardiovascular Cell therapy and Cardiovascular proteomics

Submitted by francisco.grimaldi on Mon, 08/17/2020 - 13:04
University
Faculty/school/department
SCIENCE FACULTY/ BIOMEDICINE, BIOTECHNOLOGY AND PUBLIC HEALTH/ BIOCHEMISTRY AND MOLECULAR BIOLOGY
Size of the team
number of researchers number of supporting staff number of PhD students
7
0
0
Composition of Joint Unit of Research, if relevant

Our group is mainly focused on evaluate the potential of using cell therapy in cardiovascular diseases, mainly focused on atherosclerosis, with endothelial progenitor cells and regulatory T cells. We aim to understand how these cells react, at molecular and functional level, under pathological conditions, in order to modulate them and potentiate theri regenerative and modulatory role against the inflammatory and ischemic conditions, as in atherosclerosis. We perform assays in vitro, at cellular level, and also in vivo assays administrating these cells to the CLI mice to test the response of the cells and also the ischemic tissue changes related. We also collaborate with Rexgenero Biosciences, performing cell biodistribution assays, to identify cell localization within the injured tissues or in other organs. Finally, we are currently performing an study to identify potential markers in dyslipemic/atherosclerotic patients by proteomic strategies, in order to be able to define a protein panel for early diagnosis.

PI
PI name
Mª CARMEN DURAN RUIZ
PI bio

ORCID ID: 0000-0002-1700-0141

Dr. Duran is actually associate professor of Biochemistry, and she has been working since 2013 at the University of Cadiz (UCA), teaching at both un-dergraduate and Master students, mainly Biotechnology and Biomedicine related studies. As researcher, she has more than 18 years of experience in the application of proteomics applied to biomedical research, mainly in cardiovascular diseases but also in cancer. She got her Degree and PhD in Biochemistry at the Complutense University, Madrid (2000-2004) and she completed her training at the University of Southern Denmark (2003-2004, with a Marie Curie Trainee Fellowship) and as a post-doctoral researcher at the University College, London (2005-2009). He has wide experience in the application of proteomic and mass spectrometry tools, but also has knowledge in several techniques for protein validation and further characterization (interference RNA, flow cytometry, fluorescence microscopy).
DR. Duran has got funding since 2013, from national (PI12-2680, PI16-00784, ISCIII), european(XS-00158, XS-00229, 7º EU Framework), and autonomic/FEDER (PI0026-2017, ITI-Cadiz), mainly focused on the application of cell therapy in cardiovascular diseases, atherosclerois and critical limb ischemia mainly. Actually, she is the IP of a group registered at the INIBICA (IN-CO23) and also as a PAIDI group (CTS1076, under evaluation). She has a patent accepted at a national level, related to the use of atherosclerotic secreted factors as a source to explain activation of circulating cells such as EPC (P201500877). She also got funding for equipments at the MICINN 2018 (>800.000 euros, EQC2018-004982-P y EQC2018-004871-P). She also collaborates with Rexgenero Biosciences, a company focused on the application of Cell therapy in critical limb ischemia, we are currently testing the efficiency of their product Rex-001 in a murine model of CLI. Dr. Durán has published more than 30 papers in JRC, most of them as first or last, corresponding author, and 5 book chapters. Total number of citation: 643/620, H-index: 12 (web of science)

Contact person and e-mail
Contact person
Mª CARMEN DURAN RUIZ
Contact person e-mail
WWW
Short description of research profile

Cell therapy in Critical limb ischemia
Proteomics in the search of cardiovascular research related biomarkers
Cell therapy in Atherosclerosis

Publications

Representative publications

1. Identification of the initial molecular changes detected in response to Circulating Angiogenic Cells-mediated therapy in critical limb ischemia Beltran L, Jimenez-Palomares M, Torres-Rojas M, Perez-Segura MC, Serrano A, S Gomar I, Rosal-Vela A, Eslava S, Antequera-González B, Alonso-Piñero JA, González-Rovira A, Rodriguez-Piñero M, Moreno-Luna R, Durán-Ruiz MC Stem Cell Research and Therapy, Vol 11:106
2. Molecular signatures of atheroscleroti plaques: An up-dated panel of protein related markers Eslava-Alcon S, Extremera-García MJ, González-Rovira A, Rosal-Vela A, Rojas-Torres M, Sanchez-Gomar I, Jiménez-Palomares M, Antequera-González B, Alonso-Piñero JA, Conejero R, Doiz E, Olarte J, Foncubierta-Fernández A, Lozano E, Rodríguez-Piñero M, Álvarez-Llamas G, Durán-Ruiz MC. Journal of Proteomics. 2020. April 1;221:103757
3. N_Glycosylation profile analysis of trastuzumab biosimilar candidates by Normal Phase Liquid Chromatography and MALDI-TOF MS approaches Sanchez-De Melo, I, Grassi P, Ochoa F, Bolivar J, García-Cozar, FJ; Durán-Ruiz MC. Journal of Proteomics Vol 127 (Pt B):225-33
4. RhoB controls endothelial barrier recovery by inhibiting Rac1 trafficking to the cell border Marcos-Ramiro B, García-Weber D, Barroso S, Feito J, Ortega  MC, Cernuda-Morollón E, Reglero-Real N, Fernández-Martín L, Durán MC, Alonso MA, Correas I, Cox S, Ridley AJ, Millán J. J Cell Mol Cardiol, vol 105: 12-23
5. The atheroma plaque secretome stimulates the mobilization of endothelial progenitor cells ex vivo Vega FM, Gautier V, Fernández-Ponce C, Extremera MJ, Altelaar AFM, Millan J, Tellez JC, Hernandez-Campos JA, Conejero R, Bolivar J, Pardal R, Garcia-Cózar FJ, Aguado E, Heck A JR, Duran-Ruiz MC J Cell Mol Cardiol, vol 105: 12-23

Link to extended list of publication

Technology Expertise

- Proteomic tools for the identification of differential proteins, as well as label-free quantitative approaches by mass spectrometry (MaxQuant, Proteome-Discoverer, Perseus). Software for functional classification: Ingenuity, String, cytoskape....
- Cell culture, isolation, culture and characterization of primary cells (Tcell, EPC), and cell lines. Flow Citometry and Fluorescence Microscopy
- Immunohistochemistry and immunocytochemistry
-RT-qPCR, western-blot, ELISA tools for expression analysis
- Animal research: Femoral ligation, blood flow measurements, Critical limb ischemia model, cell biodistribution assays