Phytotoxicity of chemical contaminants of the water environment: physiological, biochemical and molecular responses of plants and green algae to pharmaceuticals, herbicides and polycyclic aromatic hydrocarbons. Factors influencing the cell cycle of the green alga Chlamydomonas reinhardtii. Transient and stable transformation of selected plant and green algae species: production of recombinant proteins, lipids and waxes.
chlorophyll a fluorescence analysis in vivo (plants and alage); mitochondrial membrane potential analysis in vivo in algae cells; oxygen evolution/consumption in alagae cells suspension; antioxidative enzymes activity assesment in plant/algae material (spectrofotometry, native-PAGE); DNA/RNA isolation (plant/algae material); PCR and Real-Time PCR; proteins analysis by Western-blotting with immunodetection; lipids analysis (GC, TLC); transient and stable transformation of plants and alage