- APPROACH TO PRECISION CARDIOVASCULAR MEDICINE.
- APPLICATION OF TRANSCRIPTOMICS IN THE DIAGNOSTIC-THERAPEUTIC APPROACH TO FAMILIAL DILATED CARDIOMYOPATHY
- MOLECULAR APPROACH TO FAMILIAL DYSLIPIDEMIAS.
- FHh and SDR. HYPERKYLCHRONEMIA
- DIAGNOSTIC-THERAPEUTIC MARKERS IN MUSCLE CELL CULTURES IN FAMILIAL DILATED MYOPATHY AND DYSLIPIDEMIAS
ELECTROCARDIOGRAPH.
ECHOCARDIOGRAPHY.
CARDIO TC.
CARDIAC MRI.
ERGOMETRY.
VASCULAR ECODOPPLER.
MOLECULAR ENGINEERING AND SYSTEMS BIOLOGY.
BIOMEDICINE AND MOLECULAR ONCOLOGY.
MOLECULAR DIAGNOSIS.
• Primary cell culture techniques and immortalized cell lines. Freezing, thawing, maintenance, differentiation, and cell characterization.
• Molecular Biology techniques for modulating gene expression: transfection of plasmids and miRNAs, restriction enzymes, isolation of DNA, RNA (coding and non-coding) and proteins, RT-PCR, qPCR, PCR, electrophoresis, Western Blot, protein immunoprecipitation, gene silencing.
• Biochemistry techniques: tests for the oxidation of fatty acids (explaining tissues and cells), tests for enzymatic activities, ELISA, tests for mitochondrial capacity, or cellular bioenergetics (Seahorse).
• Cell Biology Techniques: immunocytochemistry, immunohistochemistry, flow cytometry, SORTER, cell fractionation, electroporation, fluorescent and confocal microscopy, cell phenotype assays (Seahorse).
• Advanced techniques of non-coding transcriptomic signatures.Transcriptomic and Bioinformatic techniques available to evaluate the circulating profile of non-coding RNAs, as well as the possible limitations in cardiovascular diseases and precision medicine.