CTS347: MOLECULAR BASIS OF ATHEROSCLEROSIS

Submitted by francisco.grimaldi on Tue, 06/30/2020 - 08:12
University
Faculty/school/department
FACULTY OF MEDICINE/MEDICINE/MEDICINE
Size of the team
number of researchers number of supporting staff number of PhD students
8
0
0
PI
PI name
ALIPIO MANGAS ROJAS
PI bio

University professor, member of the Spanish Society of Internal Medicine, the Spanish Society of Arteriosclerosis (SEA), and the European Society of Arteriosclerosis (EAS). Member for many years of the Research Committee of the H. U. Puerta del Mar, Evaluator of the Technical Commissions of the FIS (cardiovascular diseases), Member of the Scientific Committee of the SEA and President, Vice President and Secretary of various National Congresses of the Spanish Society of Arteriosclerosis. Member of the Editorial Committee of the Revista Clínica e Investigación en Arteriosclerosis (Official Publication of the Spanish Society of Arteriosclerosis and of the Latin American and Iberolatin American Societies of Arteriosclerosis), closely linked to the line of research developed over all these years. She has been a member of Knowledge Assessment Agencies Committees, and currently belongs to the ACSUG. Professor linked to the University Hospital Puerta del Mar (HUPM) in Cadiz, he is responsible for the Vascular Risk Factors Unit, and the Lipid Unit. He has four recognised six-year periods of research (CNEAI), and his research activity has intensified over the last 8 years together with other members of the team. For years, he has directed several research projects and clinical trials in relation to dyslipidemia and complications of atherosclerosis, achieving relevant results in the scientific community that have led to collaboration in the drafting of several Clinical Practice Guidelines. He has recognized 8 sections of teaching activity, with a wide experience that translates into the direction of more than 30 Doctoral Theses. He has been Director of the Department of Medicine of the Faculty of Medicine of Cadiz for years, and currently belongs to the Academic Committee of the Doctorate and is Director of the Department of Medicine.
(ORCID ID: 0000-0002-5402-6674)

Contact person and e-mail
Contact person
ALIPIO MANGAS ROJAS
Contact person e-mail
WWW
Short description of research profile
  • APPROACH TO PRECISION CARDIOVASCULAR MEDICINE.
  • APPLICATION OF TRANSCRIPTOMICS IN THE DIAGNOSTIC-THERAPEUTIC APPROACH TO FAMILIAL DILATED CARDIOMYOPATHY
  • MOLECULAR APPROACH TO FAMILIAL DYSLIPIDEMIAS.
  • FHh and SDR. HYPERKYLCHRONEMIA
  • DIAGNOSTIC-THERAPEUTIC MARKERS IN MUSCLE CELL CULTURES IN FAMILIAL DILATED MYOPATHY AND DYSLIPIDEMIAS
Publications

Representative publications

1. Differential expression of circulating miRNAs as a novel tool to assess BAG3-associated familial dilated cardiomyopathy. Zaragoza C, Saura M, Hernández I, Ramirez-Carracedo R, García-García F, Zamorano JL, Mangas A, Toro R. Biosci Rep. 2019 Mar 15;39(3). pii: BSR20180934.  Print 2019 Mar 29
2. Peripheral microRNA panels to guide the diagnosis of familial cardiomiopathy Belmonte T, Mangas A, Calderon-Dominguez M, Quezada-Feijoo M, Ramos M, Campuzano O, Gomez S, Peña ML, Cubillos-Arango AM, Dominguez F, Llorente-Cortés V, de Gonzalo-Calvo D, Toro R. Transl Res. 2020 Apr;218:1-15. Epub 2020 Jan 29
3. Plasma microRNAs as biomarkers for Lamin A/C-related dilated cardiomyopathy. Toro R, Blasco-Turrión S, Morales-Ponce FJ, Gonzalez P, Martínez-Camblor P, López-Granados A, Brugada R, Campuzano O, Pérez-Serra A, Rosa Longobardo F, Mangas A, Llorente-Cortes V, de Gonzalo-Calvo D. J Mol Med (Berl). 2018 Aug;96(8):845-856. Epub 2018 Jul 14
4. Proteomic identification of putative biomarkers for early detection of sudden cardiac death in a family with a LMNA gene mutation causing dilated cardiomyopathy Izquierdo I, Rosa I, Bravo SB, Guitián E, Pérez-Serra A, Campuzano O, Brugada R, Mangas A, García Á, Toro R. J Proteomics. 2016 Oct 4;148:75-84.  Epub 2016 Jul 22
5. Relationship between endothelin-1 levels and pulmonary arterial hypertension in HIV-infected patients. Feijoo MQ, Toro R, López Vazquez de la Torre M, Lennie V, Arce C, Moreno V, Valencia E, Vispo E, Almería C, Mangas A, Carbonero LM AIDS. 2014 Nov 28;28(18):2693-9.

Link to extended list of publication

Technology Expertise

ELECTROCARDIOGRAPH.
ECHOCARDIOGRAPHY.
CARDIO TC.
CARDIAC MRI.
ERGOMETRY.
VASCULAR ECODOPPLER.
MOLECULAR ENGINEERING AND SYSTEMS BIOLOGY.
BIOMEDICINE AND MOLECULAR ONCOLOGY.
MOLECULAR DIAGNOSIS.  

• Primary cell culture techniques and immortalized cell lines. Freezing, thawing, maintenance, differentiation, and cell characterization.

• Molecular Biology techniques for modulating gene expression: transfection of plasmids and miRNAs, restriction enzymes, isolation of DNA, RNA (coding and non-coding) and proteins, RT-PCR, qPCR, PCR, electrophoresis, Western Blot, protein immunoprecipitation, gene silencing.

• Biochemistry techniques: tests for the oxidation of fatty acids (explaining tissues and cells), tests for enzymatic activities, ELISA, tests for mitochondrial capacity, or cellular bioenergetics (Seahorse).

• Cell Biology Techniques: immunocytochemistry, immunohistochemistry, flow cytometry, SORTER, cell fractionation, electroporation, fluorescent and confocal microscopy, cell phenotype assays (Seahorse).

• Advanced techniques of non-coding transcriptomic signatures.Transcriptomic and Bioinformatic techniques available to evaluate the circulating profile of non-coding RNAs, as well as the possible limitations in cardiovascular diseases and precision medicine.

Existing collaborations within the SEA-EU alliance