J. Bolívar got his PhD (1997) in Biochem & Mol Biology (BQ&MB) at the University of Cádiz (UCA) working on the search of new human autoantigens by screenings human EST libraries.
During 1999-2000 he worked on Drosophila development in Dr González-Reyes’ lab at the LMB-MRC, Cambridge, UK. He later started collaboration with Prof. María Domínguez (I. of Neuroscience, Alicante) on cancer, using Drosophila as a model organism.
In 2003 he obtained a permanent position in the UCA in BQ&MB and lead a proyect on the human autoantigen ZNF330/NOA36.
In 2012 he started a succeful collaboration with the TEP-105 group on the optimization of E. coli by metabolic engeeniering for several biotransformation processes.
ORCID ID: 0000-0002-2645-9528
Biotransformations of industrial interest using Escherichia coli
Research on human auto-antigens as markers of autoimmune disseases
Research on proteases as anti tumor targets.
PCR and qPCR
Gene expression analysis
Nucleic acids and protein electrophoresis
Wester-Blot
Indirect immunofluorescence- Fluorescece and confocal microscopy
Eucaryotic and prokaryotic cell culture
Gene cloning-Protein over-expression
Genes Knock out
Centrifugation/ultracentrifugation
Flow cytometry
Biocatalysis and Metabolic engineering
1. Metabolic engineering for the optimization of hydrogen production in Escherichia coli: A review
Valle A, Cantero D and BolivarJ
Biotechnology Advances (2019) Vol: 37: 5. Pgs: 616-633
2. A brain circuit that synchronizes growth and maturation revealed through Dilp8 binding to Lgr3.
Vallejo DM, Juarez-Carreño S, Bolivar J, Morante J, Dominguez M.
Science. 2015. Vol. 350, Issue 6262, aac6767
de la Calle ME, Cabrera G, Cantero D, Valle A, Jorge Bolivar.
Microbial Cell Factories. 2019. Vol. 20:8(1) 86
Valle A, Cabrera G, Cantero D, Bolivar, J.
Microbial Cell Factories. 2015. Vol. 14, 93
5. Metabolic engineering for the optimization of hydrogen production in Escherichia coli: A review.
Valle A, Cantero D, Bolivar J
Biotechnology Advances. 2019. Vol. 37(5). 616-633