CTS569: Study of human autoantigens

Submitted by francisco.grimaldi on Thu, 07/30/2020 - 13:05
University
University of Cadiz
Faculty/school/department
FACULTY OF SCIENCE/ BIOMEDICINE, BIOTECHNOLOGY AND PUBLIC HEALTH/ BIOCHEMISTRY AND MOLECULAR BIOLOGY
Size of the team
number of researchers number of supporting staff number of PhD students
13
0
0
PI
PI name
JORGE PEREZ BOLIVAR
PI email
jorge.bolivar@uca.es
PI bio

J. Bolívar got his PhD (1997) in Biochem & Mol Biology (BQ&MB) at the University of Cádiz (UCA) working on the search of new human autoantigens by screenings human EST libraries.
During 1999-2000 he worked on Drosophila development in Dr González-Reyes’ lab at the LMB-MRC, Cambridge, UK. He later started collaboration with Prof. María Domínguez (I. of Neuroscience, Alicante) on cancer, using Drosophila as a model organism.
In 2003 he obtained a permanent position in the UCA in BQ&MB and lead a proyect on the human autoantigen ZNF330/NOA36.
In 2012 he started a succeful collaboration with the TEP-105 group on the optimization of E. coli by metabolic engeeniering for several biotransformation processes.

ORCID ID: 0000-0002-2645-9528

Contact person and e-mail
Contact person
JORGE PÉREZ BOLIVAR
Contact person e-mail
jorge.bolivar@uca.es
WWW
https://cts569.uca.es/
Short description of research profile

Biotransformations of industrial interest using Escherichia coli
Research on human auto-antigens as markers of autoimmune disseases
Research on proteases as anti tumor targets.

Research area
MEDICAL AND HEALTH SCIENCES » Health sciences
Publications

Representative publications

1. Metabolic engineering for the optimization of hydrogen production in Escherichia coli: A review Valle A, Cantero D and BolivarJ Biotechnology Advances   (2019) Vol: 37: 5. Pgs: 616-633
2. A brain circuit that synchronizes growth and maturation revealed through Dilp8 binding to Lgr3. Vallejo DM, Juarez-Carreño S, Bolivar J, Morante J, Dominguez M. Science. 2015. Vol. 350, Issue 6262, aac6767
3. A genetically engineered Escherichia coli strain overexpressing the nitroreductase NfsB is capable of producing the herbicide D-DIBOA with 100% molar yield. de la Calle ME, Cabrera G, Cantero D, Valle A, Jorge Bolivar. Microbial Cell Factories. 2019. Vol.  20:8(1) 86
4. Identification of enhanced hydrogen and ethanol Escherichia coli producer strains in a glycerol-based medium by screening in single-knock out mutant collections. Valle A,  Cabrera G, Cantero  D, Bolivar, J. Microbial Cell Factories. 2015. Vol. 14,  93
5. Metabolic engineering for the optimization of hydrogen production in Escherichia coli: A review. Valle A, Cantero D, Bolivar J Biotechnology Advances. 2019. Vol. 37(5). 616-633

Link to extended list of publication

https://cts569.uca.es/publicaciones-en-revistas/
Technology Expertise

PCR and qPCR
Gene expression analysis
Nucleic acids and protein electrophoresis
Wester-Blot
Indirect immunofluorescence- Fluorescece and  confocal microscopy
Eucaryotic and prokaryotic cell culture
Gene cloning-Protein over-expression
Genes Knock out
Centrifugation/ultracentrifugation
Flow cytometry
Biocatalysis and Metabolic engineering