ORCID ID: 0000-0002-7232-2661
Bachelors Degree in Biological Sciences from the University of Valencia, 1990. Board Specialist in Immunology in 1996 (Hospital Reina Sofía, Córdoba). PhD in Biological Sciences from the University of Cordoba in 1997. Post-doctoral stay at the CIML (Marseille, France) 1998-2000.
Reincorporation to the University of Murcia, including "Ramón y Cajal" contract (2001-2007).
"Miguel Servet" contract, Puerto Real University Hospital (Cádiz) (2007-10).
Associate Professor of Immunology at the University of Cadiz (2010-)
38 indexed publications. Index H: 14. 1158 citations.
Our group has analyzed the regulatory functions in T-cells of the LAT and NTAL membrane adapters, as well as their relationship to the Lck kinase. Our lines of research aim to deepen the study of these functions and analyze the implications in some immune-based pathologies such as rheumatoid arthritis, since aberrant activation of T cells seems to be at the basis of the initiation and/or maintenance of this disease. Our research includes studies with cell lines, with mice, and analysis of primary lymphocytes of patients and controls.
Intracellular signaling in T-lymphocytes
- Flow cytometry
- Cell Sorting
- Cell culture
- Purification of lymphocytes, monocytes and granulocytes
- Cell activation and proliferation studies
- Intracellular signaling studies
- Protein detection and quantification
- ELISA, Western blot and immunoprecipitation
- DNA Cloning
- Directed Mutagenesis
- Transfection
- Lentiviral Transduction
- PCR and RT-PCR
- Generation of genetically modified animals
- Analysis of thymic development in mice
1. Slow phosphor-Y-LAT-ion for TCR ligand discrimination
Aguado, Enrique; Arbulo-Echevarria, Mikel M.
Nature Immunology (2019) Vol: 20 (11). Pgs: 1420-1422
Arbulo-Echevarria, MM; Narbona-Sánchez, I; Fernandez-Ponce, CM; Vico-Barranco, I; Rueda-Ygueravide, MD; Dustin, ML; Miazek, A; Duran-Ruiz, MC; García-Cózar, F; Aguado, E
FRONTIERS IN IMMUNOLOGY, 9:115
Arbulo-Echevarria, MM; Munoz-Miranda, P; Caballero-Garcia, A; Poveda-Díaz, JL; Fernández-Ponce, C; Durán-Ruiz, MC; Miazek, A; García-Cózar, F; Aguado, E.
JOURNAL OF LEUKOCYTE BIOLOGY, 100 (2): 351-360
Marek-Bukowiec, K.; Aguado, E.; Miazek, A.
GENES AND IMMUNITY, 17 (5): 313-320
García-Blesa, A; Klossowicz, M; López-Osuna, C; Martínez-Florensa, M; Malissen, B; García-Cózar, FJ; Miazek, A; Aguado, E
BIOCHEMICAL JOURNAL, 450: 511-521