Skip to main content

CTS1069. Intracellular signaling in lymphocytes and implications in immune-based pathologies

Submitted by francisco.grimaldi on

Research Areas & Expertise

MEDICAL AND HEALTH SCIENCES » Basic medical research

- Flow cytometry

- Cell Sorting

- Cell culture

- Purification of lymphocytes, monocytes and granulocytes

- Cell activation and proliferation studies

- Intracellular signaling studies

- Protein detection and quantification

- ELISA, Western blot and immunoprecipitation

- DNA Cloning

- Directed Mutagenesis

- Transfection

- Lentiviral Transduction

- PCR and RT-PCR

- Generation of genetically modified animals

- Analysis of thymic development in mice

Principal Investigator

ENRIQUE AGUADO VIDAL

enrique.aguado@uca.es

ORCID ID: 0000-0002-7232-2661

Bachelors Degree in Biological Sciences from the University of Valencia, 1990. Board Specialist in Immunology in 1996 (Hospital Reina Sofía, Córdoba). PhD in Biological Sciences from the University of Cordoba in 1997. Post-doctoral stay at the CIML (Marseille, France) 1998-2000.

Reincorporation to the University of Murcia, including "Ramón y Cajal" contract (2001-2007).

"Miguel Servet" contract, Puerto Real University Hospital (Cádiz) (2007-10).

Associate Professor of Immunology at the University of Cadiz (2010-)

38 indexed publications. Index H: 14. 1158 citations.

University
Faculty/school/department
MEDICINE/BIOMEDICINE, BIOTECHNOLOGY AND PUBLIC HEALTH/ IMMUNOLOGY
PI bio

ORCID ID: 0000-0002-7232-2661

Bachelors Degree in Biological Sciences from the University of Valencia, 1990. Board Specialist in Immunology in 1996 (Hospital Reina Sofía, Córdoba). PhD in Biological Sciences from the University of Cordoba in 1997. Post-doctoral stay at the CIML (Marseille, France) 1998-2000.

Reincorporation to the University of Murcia, including "Ramón y Cajal" contract (2001-2007).

"Miguel Servet" contract, Puerto Real University Hospital (Cádiz) (2007-10).

Associate Professor of Immunology at the University of Cadiz (2010-)

38 indexed publications. Index H: 14. 1158 citations.

Composition of Joint Unit of Research

Our group has analyzed the regulatory functions in T-cells of the LAT and NTAL membrane adapters, as well as their relationship to the Lck kinase. Our lines of research aim to deepen the study of these functions and analyze the implications in some immune-based pathologies such as rheumatoid arthritis, since aberrant activation of T cells seems to be at the basis of the initiation and/or maintenance of this disease. Our research includes studies with cell lines, with mice, and analysis of primary lymphocytes of patients and controls.

PI name
ENRIQUE AGUADO VIDAL
Short description of research profile

Intracellular signaling in T-lymphocytes

Contact person
ENRIQUE AGUADO VIDAL
Contact person e-mail
Technology Expertise

- Flow cytometry

- Cell Sorting

- Cell culture

- Purification of lymphocytes, monocytes and granulocytes

- Cell activation and proliferation studies

- Intracellular signaling studies

- Protein detection and quantification

- ELISA, Western blot and immunoprecipitation

- DNA Cloning

- Directed Mutagenesis

- Transfection

- Lentiviral Transduction

- PCR and RT-PCR

- Generation of genetically modified animals

- Analysis of thymic development in mice

1. Slow phosphor-Y-LAT-ion for TCR ligand discrimination

Aguado, Enrique; Arbulo-Echevarria, Mikel M.

Nature Immunology   (2019) Vol: 20 (11). Pgs: 1420-1422

2. A Stretch of Negatively Charged Amino Acids of Linker for Activation of T-Cell Adaptor Has a Dual Role in T-Cell Antigen Receptor Intracellular Signaling

Arbulo-Echevarria, MM; Narbona-Sánchez, I; Fernandez-Ponce, CM; Vico-Barranco, I; Rueda-Ygueravide, MD; Dustin, ML; Miazek, A; Duran-Ruiz, MC; García-Cózar, F; Aguado, E

FRONTIERS IN IMMUNOLOGY, 9:115

3. Non-T cell activation linker (NTAL) proteolytic cleavage as a terminator of activatory intracellular signals

Arbulo-Echevarria, MM; Munoz-Miranda, P; Caballero-Garcia, A; Poveda-Díaz, JL; Fernández-Ponce, C; Durán-Ruiz, MC; Miazek, A; García-Cózar, F;  Aguado, E.

JOURNAL OF LEUKOCYTE BIOLOGY, 100 (2): 351-360

5. The membrane adaptor LAT is proteolytically cleaved following Fas engagement in a tyrosine phosphorylation-dependent fashion

García-Blesa, A; Klossowicz, M; López-Osuna, C; Martínez-Florensa, M; Malissen, B; García-Cózar, FJ; Miazek, A; Aguado, E

BIOCHEMICAL JOURNAL, 450: 511-521

number of researchers
7
number of supporting staff
0
number of PhD students
0
Size of the team