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Capillary electrophoresis P/ACE MDQ plus SCIEX with LIF 488/520, 260/320 nm detectors

Submitted by Mucha on
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Type of availability
Kind of shared resource
Category of shared resource
Shareable
Yes
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Location

Location

Laboratory of Biologically Active Compounds Chemistry, Department of Molecular Biochemistry, Faculty of Chemistry, University of Gdansk, Gdansk, Poland

Contact person and e-mail
Contact person
dr Piotr Mucha
Contact person e-mail
Short description of shared resource

Capillary electrophoresis (CE) is an electrokinetic separation method that uses a narrow capillary tube filled with an electrolyte buffer to separate charged molecules based on their differential migration in an electric field. CE achieves high-resolution separation of complex mixtures of molecules of different sizes like organic and inorganic ions, amino acids, alkaloids, plant metabolites, hormones, peptides, proteins or DNA/RNAs with high speed, sensitivity, and efficiency, while requiring minute sample volumes and consuming few reagents. The use of a fluorescence detector with excitation at 488 nm enables high-sensitivity analysis of fluorescein-labeled compounds. Excitation at 260 nm, on the other hand, enables the analysis of native/unlabeled compounds containing tryptophan like peptides or  proteins.       

Methods and Expertise

We have extensive experience in the electrokinetic analysis of low- and high-molecular compounds and its interactions using capillary electrophoresis (CE). We work with both synthetic samples derived from chemical synthesis and samples of biological origin. It is possible to analyze both ionic compounds and electrically neutral molecules (e.g., in the presence of SDS). In terms of size, our interests range from small objects (simple organic and inorganic ions) to large objects such as protein, DNA/RNA or whole viruses. 

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Details

Labels/certification

Labels/certification

The equipment is used for laboratory testing. We do not have any special certificates.

Potential application

Potential application

The potential scope of application includes qualitative and quantitative analysis of biomolecules. Virtually any type. When using a 488 nm LIF detector, prior derivatization (with fluorescein) is necessary. However, for excitation at 260 nm, the presence of tryptophan is sufficient. 

Rules of access to the shared resource

Proposed rules of access to the shared resource

We offer shared use of equipment for scientific research on the basis of co-authorship in publications resulting from its use.