Laboratory of imaging and data analysis

Submitted by awroblewska on Mon, 01/10/2022 - 09:44
Type of availability
Kind of shared resource
Category of shared resource


  • Institute of Biotechnology, Abrahama 58, 80-307 Gdańsk, Poland, Core Facility, room 313, and
  • Builiding of the Tri-City University Animal House – Research Service Centre, Medical Univeristy of Gdańsk, Dębinki 1, 80-211 Gdańsk, Poland, room 117
Contact person and e-mail
Contact person
Rafał Sądej, PhD
Contact person e-mail
Short description of shared resource

Laboratory of imaging and data analysis offers a wide range of imaging possibilities of various objects. The laboratory specializes in imaging alive or fixed biological preparations in visible and fluorescent light. Additionally, a variety of analyzes of the obtained images can be performed in the laboratory. The laboratory is equipped with several high-class microscopes: inverted fluorescence microscope - NIKON Eclipse TE300 with Hamamatsu ORCA C4742 camera, simple fluorescence microscope - OLYMPUS BX51 with FView camera, fluorescent stereoscopic microscope - LEICA MZ10F, fluorescence holotomographic microscope - TOMOCUBE-2, and scanning confocal microscope with white laser - LEICA SP8 X.

Scientific disciplines

Potential application

Potential application

NIKON PCM2000 confocal scanning microscope is equipped with three lasers with wavelengths 488, 546 and 633nm, a HBO mercury lamp and an ultra-sensitive Hamamatsu Orca C4742 color camera. The system allows to collect images with very low fluorescence intensity. Available lenses: 4x / 0.13, 10x / 0.30, 20x / 0.45, 40x / 060, 63x / 1.4 oil immersion, 100x / 1.4 oil immersion. It enables imaging in bright field, phase and Nomarski contrast, in superfluous light and fluorescence.

Scanning confocal microscope LEICA SP8X with white laser. The white laser enables the selection of any wavelength of the excitation light with an accuracy of 1 nm in the range from 470 nm to 670 nm. Additionally, the microscope is equipped with an argon laser with five excitation lines (458, 476, 488, 496, 514 nm) and a 405 nm diode laser. As a result, the system can be fully adapted to the exact excitation parameters of the various fluorescent dyes. The system is equipped with an in vivo observation chamber, which enables long-term observations of fluorescence in living cells. It enables imaging in bright field, phase contrast, transient light and fluorescence. There is possibility to analyze the intensity of fluorescence, co-localization, FRET  and FRAP.…

Scanning confocal microscope Leica HCS LSI is designed for viewing 'large' specimens. The maximum field of view is 484mm2, it is possible to combine images. The microscope is equipped with four diode lasers (405, 488, 561, 635nm) for confocal operation, a white light source (EPI) and a UV light source. While working in the confocal mode, the image is recorded by a single spectral detector with a working range of 430-750nm and spectral resolution of 5nm. In the operating mode of the stereomicroscope, the image can be viewed directly by the operator or using a monochrome CCD camera. The microscope is equipped with filters for excitation of classic dyes or fluorophores (DAPI, GFP, FM-54). Available micro-lenses: 10x / 0.30, 40x / 1.15 oil immersion and 64x / 1.30 oil immersion. The system is equipped with an in vivo observation chamber, which enables long-term observations of fluorescence in living cells.…

Rules of access to the shared resource

Proposed rules of access to the shared resource

Access to the equipment is possible on the basis of free scientific cooperation.