Mass Spectrometry Laboratory

Submitted by awroblewska on Mon, 01/10/2022 - 10:49
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Type of availability
Kind of shared resource
Category of shared resource
Shareable
Yes
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Location

Location

Institute of Biotechnology, Abrahama 58, 80-307 Gdańsk, Poland, Core Facility, room P17

Contact person and e-mail
Contact person
Paulina Czaplewska, PhD
Contact person e-mail
Short description of shared resource

The laboratory is equipped with three AB Sciex mass spectrometers: MALDI TOF / TOF 5800, TripleTOF 5600+, QTRAP 6500, as well as the CESI 8000 Plus (Sciex) capillary electrophoresis system. 

Details

Potential application

Potential application

MALDI TOF/TOF 5800+ mass specrometercan work in linear and reflection mode, as well as in MS / MS mode. The wide measuring range enables mass measurements from approx. 100 Da to approx. 200 kDa.Proteomics, It can be used in MALDI imaging and identification of compounds.

TripleTOF 5600+ mass specrometer is a hybrid system perfect for both the qualitative analysis of proteins, peptides and small molecules, as well as for quantitative analysis. The combination of high resolution, detection sensitivity with quick sample analysis and stable mass calibration allows the use of this model for many applications: proteomics, metabolomics, quantitative and qualitative analyzes, MRM , IDA, peptide and protein sequencing, SWATH analyzes.

Integrated Capillary Electrophoresis and Ionization System Optims Cesi 8000 Plus (2016) combines the advantages of CE with the ability to analyze the separated analytes using electrospray. In our Laboratory, this system is dedicated to the Triple TOF 5600+ spectroemeter. In such a combination, it is perfect for use in proteomics, metabolomics, chemical analysis, glycomics, characterization of protein interactions, and identification of protein modifications. Our instrument is equipped with 4 UV filters which allows for classic CE analyzes. It is also possible to expand the system with additional detectors such as LIF or PDA.

Liquid chromatograph with tandem mass spectrometry QTRAP 6500 is equipped with a triple quadrupole, in which the second analyzer can work interchangeably as a quadrupole and an ion trap. It is therefore characterized by high sensitivity and enables operation in the following modes: Q1 MS, Q3 MS, Product Ion, Precursor Ion, Neutral Loss or Gain, MRM, EMS, EPI, ER, MS3, MRM3, TripleTrap Scanning. Perfect for metabolite identification, MRM based quantification. It provides excellent selectivity and measurement speed. In the case of this spectrometer, it is possible to record MS3 spectra for i.a. quantitative analyzes.It enables separation of mixtures and quantification of compounds, application in proteomics and metabolomics.
 

Rules of access to the shared resource

Proposed rules of access to the shared resource

Access to the equipment is possible on the basis of free scientific cooperation.