Laboratory of Biomolecular Interactions

Submitted by awroblewska on Mon, 01/10/2022 - 11:15
Type of availability
Kind of shared resource
Category of shared resource



Institute of Biotechnology, Core Facility, Abrahama 58, 80-307 Gdańsk, Poland, room P18

Contact person and e-mail
Contact person
Rafał Sądej, PhD
Contact person e-mail
Short description of shared resource

The Laboratory of Biomolecular Interactions provides access to a wide range of equipment for studying complex molecular interactions in isolated biochemical systems, whole cells and complex biological systems. The laboratory equipment includes BioScope Resolve Atomic Force Microscope, High-Speed Atomic Force Microscope MS-NEX, Biacore T200 biosensor system, Monolith NT.115 and Circular Dichroism Spectrometer Jasco J-1500.


Potential application

Potential application

The BioScope Resolve Atomic Force Microscope with the MultiMode8 Lockout Specs scanning head is a unique AFM system that enables high-resolution observation of complex biological systems, whole cells and isolated biochemical systems; observation and analysis of systems composed of single proteins, complexes of nucleic acids and proteins as well as isolated multicomponent enzymatic systems. This microscope enables high-resolution imaging of very small molecules as well as large eukaryotic cells. It allows you to observe preparations fixed as well as particles in aqueous solutions.

High-Speed Atomic Force Microscope MS-NEX enables high-resolution imaging of biological samples in a very short time (20 frames / sec - 50 ms / frame - 2000 lines / sec) in aqueous solutions. Ultrafast imaging enables the observation of dynamic processes occurring in the analyzed research systems in real time. The device enables the real-time analysis of the interactions of single protein molecules and nucleic acids in solution, the study of the kinetics of native particles, analysis of preparations at the cellular and tissue level, aimed at identifying areas undergoing dynamic structural changes. It is also possible to analyze the activity of plant, animal and human pathogens, to analyze changes in infected tissues and to study the structure of the bacterial biofilm.

Biacore T200 biosensor system enables analysis of the interactions (from small molecules to viruses and cells) in real time without the need to label the sample. Only a small sample volume (50-350ul), at a low concentration (> 10 pM) is needed. The high sensitivity of the Biacore T200 equipment allows for the analysis of interactions in the range of constants: range of measurement of the association constants from 10^3 to 3 × 10^9 M^ -1s^-1; affinity measurement range fM - mM.

Monolith NT.115 can be used to measure the affinity (KD, dissociation constant) between selected molecules in a solution at a given temperature (22-45⁰C), study membrane proteins associated with liposomes or in a solution containing detergents, analysis of multi-component reactions, the order of formation of complex complexes and the study of factors influencing such structures, distinguishing aggregation and other artifacts from specific interactions, or to study stoichiometry and determine the number of binding sites of given molecules.

 Circular Dichroism Spectrometer Jasco J-1500 can be used to assess the secondary structure of proteins and to analyze protein denaturation.

Rules of access to the shared resource

Proposed rules of access to the shared resource

Access to the equipment is possible on the basis of free scientific cooperation.