Barocycler 2320EXT

Submitted by awroblewska on Wed, 01/12/2022 - 09:11
Gallery
Barocycler 2320EXT
Attributes
Type of availability
research and commercial
Kind of shared resource
infrastructure
Category of shared resource
large
Shareable
Yes

Location

University
University of Gdańsk
Location

International Centre for Cancer Vaccine Science, Laboratory of mass spectrometry, Kładki 24, 80-822 Gdańsk, Poland, room 315

Contact person and e-mail
Contact person
Sachin Kote, PhD
Contact person e-mail
sachin.kote@ug.edu.pl
Short description of shared resource

The instrument uses Pressure Cycling Technology (PCT) based on repeated cycles of hydrostatic pressure between ambient (14.7 psi) and ultra-high levels (up to 45,000 psi). These rapid changes in pressure are used to control biomolecular interactions for applications such as accelerated proteolytic digestion and improved extraction of cellular  components,  including  proteins  and  lipids.  The  PCT  platform  allows  for  a  high  degree  of  safety, speed, reproducibility, and convenience in modern sample preparation protocols.

http://www.pressurebiosciences.com/downloads/sell-sheets/Barocycler-NEP2320.pdf

Scientific disciplines
(CQ) BIOCHEMISTRY & MOLECULAR BIOLOGY
Scientific Themes (keywords)
sample preparation
proteolytic digestion
extraction of cellular components
Pressure Cycling Technology
mass spectrometry
cell lysis
Details

Potential application

Potential application

Application includes: extraction and recovery of more membrane proteins, improved protein digestion, differential lysis in a mixed sample base, pathogen inactivation, increased DNA detectability, control of the sample preparation process.