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Microbial Biochemistry and Immune Mechanisms

Submitted by Karolina Stojo… on

Research Areas & Expertise

NATURAL SCIENCES » Biological sciencesMEDICAL AND HEALTH SCIENCES » Basic medical researchMEDICAL AND HEALTH SCIENCES » Clinical medicine
  • PCR, qPCR
  • Flow cytometry
  • Molecular cloning
  • Bacterial genome and plasmid modification
  • Quantitative analyses of gene and protein expression.
  • Proteomic approaches
  • Cell culture (bacterial and mammalian)
  • Immunofluorescence
  • Immunohistochemistry
  • Fluorescent microscopy
     

 

Principal Investigator

Faculty/school/department
Faculty of Biology/Department of General and Medical Biochemistry
Composition of Joint Unit of Research
  • Bacterial physiology and persister cell biology – studying antibiotic tolerance, cell cycle, biochemical pathways, and mechanisms of bacterial survival and eradication.
  • Bacterial molecular biology and genetic engineering – including genome modification, plasmid construction, cloning, and molecular analyses of gene expression.
  • Immunology of host cells in diabetic kidney disease – focusing on Fcγ receptor signaling and regulation in podocytes and phagocytic cells under physiological and hyperglycaemic conditions.
PI name
Ewa Laskowska
Short description of research profile
  • Bacterial physiology and persister cell biology
  • Mechanisms of antibiotic tolerance and resistance
  • Bacterial genetic engineering and genome modification, and molecular analyses
  • Host immune regulation in diabetic kidney disease
  • Fcγ receptor signaling in podocytes and phagocytes
  • Molecular and cellular analysis of host–pathogen interactions

     

Contact person
Ewa Laskowska
Contact person e-mail
Technology Expertise
  • PCR, qPCR
  • Flow cytometry
  • Molecular cloning
  • Bacterial genome and plasmid modification
  • Quantitative analyses of gene and protein expression.
  • Proteomic approaches
  • Cell culture (bacterial and mammalian)
  • Immunofluorescence
  • Immunohistochemistry
  • Fluorescent microscopy
     

 

Stojowska-Swędrzyńska K., Kuczyńska-Wiśnik D., Laskowska E. Influence of Nε-lysine acetylation on the formation of protein aggregates and antibiotic persistence in E. coli. Molecules. 2024 Jan 12;29(2):383.

doi: 10.3390/molecules29020383

Łupkowska A., Monem S., Dębski J., Stojowska-Swędrzyńska K., Kuczyńska-Wiśnik D., Laskowska E. Protein aggregation and glycation in Escherichia coli exposed to desiccation-rehydration stress. Microbiol Res. 2023 May:270:127335.

doi: 10.1016/j.micres.2023.127335

 

Stojowska-Swędrzyńska K., Laskowska E., Kuczyńska-Wiśnik D. Molecular Basis of Persister Awakening and Lag-Phase Recovery in Escherichia coli After Antibiotic Exposure. Int J Mol Sci. 2026 Jan 1;27(1):467.

doi: 10.3390/ijms27010467

Typiak M., Kulesza T., Rachubik P., Rogacka D., Audzeyenka I., Angielski S., Saleem M.A., Piwkowska A. Role of Klotho in hyperglycemia: its levels and effects on fibroblast growth factor receptors, glycolysis, and glomerular filtration. Int J Mol Sci. 2021 Jul 23;22(15):7867.

doi: 10.3390/ijms22157867 
 

Typiak M., Piwkowska A. Antiinflammatory Actions of Klotho: Implications for Therapy of Diabetic Nephropathy. Int. Int J Mol Sci. 2021 Jan 19;22(2):956.

doi: 10.3390/ijms22020956
 

Size of the team
from 4 to 7
number of researchers
5
number of supporting staff
2
number of PhD students
1
Size of the team