Main focus on biochemical characteristic of proteolytic enzymes using combinatorial chemistry methods. Design and synthesis of selective substrates of particular protease. Main interest: neutrophil proteases, diagnostic, molecular probes ect. Using our techniques we are able to characterized substrate specificity of any proteolytic enzyme. Also we are involoved in antimcrobial and immunostimulating peptides.
A Peptidomimetic Fluorescent Probe to Detect the Trypsin β2 Subunit of the Human 20S Proteasome.
Wysocka M, Romanowska A, Gruba N, Michalska M, Giełdoń A, Lesner A.Int J Mol Sci. 2020 Mar 31;21(7):2396. doi: 10.3390/ijms21072396.
Korkmaz B, Lesner A, Wysocka M, Gieldon A, Håkansson M, Gauthier F, Logan DT, Jenne DE, Lauritzen C, Pedersen J.Biochem Pharmacol. 2019 Jun;164:349-367. doi: 10.1016/j.bcp.2019.04.006.
Popow-Stellmaszyk J, Bajorowicz B, Malankowska A, Wysocka M, Klimczuk T, Zaleska-Medynska A, Lesner A.Bioconjug Chem. 2018 May 16;29(5):1576-1583. doi: 10.1021/acs.bioconjchem.8b00100.
Guarino C, Gruba N, Grzywa R, Dyguda-Kazimierowicz E, Hamon Y, Łȩgowska M, Skoreński M, Dallet-Choisy S, Marchand-Adam S, Kellenberger C, Jenne DE, Sieńczyk M, Lesner A, Gauthier F, Korkmaz B.J Med Chem. 2018 Mar 8;61(5):1858-1870. doi: 10.1021/acs.jmedchem.7b01416.
Bladder cancer detection using a peptide substrate of the 20S proteasome.
Gruba N, Wysocka M, Brzezińska M, Dębowski D, Sieńczyk M, Gorodkiewicz E, Guszcz T, Czaplewski C, Rolka K, Lesner A.FEBS J. 2016 Aug;283(15):2929-48. doi: 10.1111/febs.13786. Epub 2016 Jul 14.