orcid.org/0000-0002-1099-8885
Our research group focuses on diseases related to the aggregation of polypeptides and proteins. We study the aggregation process itself using fluorescence and electrophoretic methods, size-exclusion chromatography, and LC-MS coupled with enzymatic digestion. We are also deeply involved in the search for aggregation inhibitors as well as in studying the role that the 20S proteasome, stimulated by the activators we design, may play in removing toxic soluble oligomers. We evaluate the activation capacity of the modulators both in vitro, using the proteasome isolated from human erythrocytes, and in cells transfected with plasmids encoding aggregation-prone proteins.
synthesis of peptides and peptidomimetics
organic synthesis
HPLC
FTIR
protein crystallography
electrophoresis
Osmulski Paweł A., Karpowicz Przemysław, Jankowska Elżbieta, Bohmann Jonathan, Pickering Andrew M., Gaczynska Maria
New peptide-based pharmacophore activates 20S proteasome
Molecules 25: 1-17, 1439 (2020)
DOI:10.3390/molecules25061439
Sandra Skibiszewska, Szymon Żaczek, Agnieszka Dybala-Defratyka, Katarzyna Jędrzejewska, Elżbieta Jankowska
Influence of short peptides with aromatic amino acid residues on aggregation properties of serum amyloid A and its fragments.
Arch. Biochem. Biophys. 681: 108264-108272 (2020)
DOI: 10.1016/j.abb.2020.108264
Małgorzata Giżyńska, Julia Witkowska, Przemysław Karpowicz, Rafał Rostankowski, Estrella S. Chocron, Andrew M. Pickering, Pawel Osmulski, Maria Gaczynska, Elżbieta Jankowska
Proline- and Arginine-Rich Peptides as Flexible Allosteric Modulators of Human Proteasome Activity
J. Med. Chem. 62: 359-370 (2019)
DOI: 10.1021/acs.jmedchem.8b01025
Witkowska, J.,Gizynska, M.,Grudnik, P.,Golik, P.,Karpowicz, P.,Giedon, A.,Dubin, G., Jankowska, E.
Crystal structure of a low molecular weight activator Blm-pep with yeast 20S proteasome - insights into the enzyme activation mechanism
Sci Rep 7: 6177 (2017)
DOI: 10.1038/s41598-017-05997-4
Orlikowska M., Szymańska A., Borek D., Otwinowski Z., Skowron P., Jankowska E.
Structural characterization of V57D and V57P mutants of human cystatin C, an amyloidogenic protein
Acta Cryst. D69, 577-586 (2013)
DOI: 10.1107/S0907444912051657